Search results for "molecular tool"

Fusarium wilt of cyclamen : early detection and biocontrol

2016

Fusarium wilt of cyclamen is one of the most damaging diseases of cyclamen. The causal agent, Fusarium oxysporum f. sp. cyclaminis, is a soil-borne fungus. Losses can reach more than 50 % of the production. Several methods of control are available, but none of them offer an efficient and environmentally friendly solution. In this context, a project was developed in collaboration with the French institute of horticulture, Astredhor, which represents the producers, the INRA of Dijon, for its expertise on F. oxysporum and the company Agrene for its expertise in biological control. The project has two goals: i) design a molecular marker specific of Fusarium oxysporum f. sp. cyclaminis allowing …

Des outils moléculaires toujours plus multifonctionnels pour l’étude de structures alternatives d’acides nucléiques.

2022

G-Quadruplexes (G4) are non-canonical secondary structure found throughout the genome and transcriptome in guanine rich sequences. Their involvement in biologically relevant processes (telomeres maintenance, transcription and translation) has been demonstrated, yet their role is still not fully elucidated. To investigate their role, we focus on the development of biomimetic molecular tools that could be used either to isolate G4 by new generation sequencing techniques or visualize them directly in cell. This class of molecular tools (TASQ: Template Assembled Synthetic Quartet) is composed by four guanine moieties linked to a central template thanks to spacers of different chemical nature. I…

Molecular tools to monitor a biocontrol agent and to assess its environmental impacts

2008

The use of non pathogenic strains of Fusarium oxysporum has been proposed to control Fusarium diseases responsible for severe damages in many crops of economical importance. In order to monitor the biocontrol strain Fo47 after application in greenhouse and field, we have developed a specific molecular tool based on a sequence characterized amplified region (SCAR). Primers targeting a 210-bp fragment were designed to set up a real time PCR assay allowing the identification of the biocontrol strain and its quantification in environmental samples. The assay enabled specific detection of the strain in soils without cross detection of autochthonous populations of F. oxysporum. After its introduc…

Microbial Biogeography at the scale of France by the use of molecular tools applied to the French soil quality monitoring network (RMQS)

2008

Description and phylogenetic relationships of a new species of treefrog of theOsteocephalus buckleyispecies group (Anura: Hylidae)

2020

The Osteocephalus buckleyi species group is widely distributed in primary and secondary forests of the Amazon Basin and Guiana Region. Based on integrative analysis, including morphological and genetic data, we estimate the phylogenetic relationships and species boundaries among populations of the Osteocephalus buckleyi group from the Ecuadorian Amazon, focusing on the O. verruciger-O. cannatellai species complex. Our results uncovered the existence of one confirmed candidate species from Sangay National Park and one unconfirmed candidate species. Here, we describe the new species which is morphologically and ecologically distinct from other Osteocephalus species. The new species is unusual…